General Instructions for Grape Sample Collection

Appropriate sampling techniques are critical for reliable diagnosis of viruses. Depending on the time of year, either leaf or cane samples can be taken.

If there are no symptoms of disease, gather a random sample of either whole-leaf or can segments using a uniform pattern through the vineyard, such as a “W” or “X” pattern.

If suspect virus symptoms are present, gather samples from affected and adjacent-to-affected vines.

Leaf Sampling

  1. Collect six to eight mature leaves with petioles (leaf stems) from different parts of the grapevine. Use a plastic bag like a glove and drop the leaves into the bag and seal.
  2. Label individual bags and document location of vines in the vineyard block with cultivar name and other pertinent information.
  3. Keep the samples cool until transportation to the lab, in a cooler or refrigerator. Do not freeze.

Cane Sampling

  1. Collect canes randomly from the grapevine. Samples should be collected throughout the vine (i.e., both cordons or arms) and be at least two or three internodes in length. Place samples in a zip top plastic bag.
  2. Label individual bags and document location of vines in the vineyard block with the cultivar name and other pertinent information.